EFEKTIVITAS DAUN SIRIH MERAH (Piper crocatum) PADA SANITASI DI RUANG PENAMPUNGAN SUSU

Eulis Tanti Marlina(1*), Ellin Harlia(2), Yuli Astuti Hidayati(3), Deden Zamzam Badruzzaman(4)

(1) Laboratorium Mikrobiologi dan Penanganan Limbah, Departemen Teknologi Hasil Peternakan, Fakultas Peternakan, Universitas Padjadjaran
(2) Laboratoriun Mikrobiologi dan Penanganan Limbah Dep. Teknologi Hasil Peternakan Fapet Unpad
(3) Laboratoriun Mikrobiologi dan Penanganan Limbah Dep. Teknologi Hasil Peternakan Fapet Unpad
(4) Laboratoriun Mikrobiologi dan Penanganan Limbah Dep. Teknologi Hasil Peternakan Fapet Unpad
(*) Corresponding Author

Sari


Red betel is a multipurpose plant, because apart from being an ornamental plant, because of the beauty of its leaves, it also has several active compounds as antibacterial agents. The aim of this research was to study the effectiveness of red betel leaf extract as a natural disinfectant in the milk storage room through its inhibitory power against bacteria in situ and its effect on reducing the number of total bacteria on the floor, table, and walls in the milk storage room.     The research used experimental method using completely randomized design with 3 treatments of red betel leaf extract concentration, P1 = 15%, P2 = 25%, P3 = 35%, each treatment was repeated 6 times. The research data were analyzed using analysis of variance and Tukey's test. The results showed that the highest reduction in the total number of bacteria was in the red betel leaf extract with a concentration of 35%, both on the floor, table, and walls in the milk storage room, respectively 52.40%, 59.96%, and 61.77%. The inhibition zone of red betel leaf extract against bacteria in situ was the highest at a concentration of 35% red betel leaf extract on the floor, table and wall, namely 9.12; 10.65; and 10.76 mm.


Kata Kunci


daun sirih merah, disinfektan, total bakteri, zona hambat

Teks Lengkap:

PDF

Referensi


Agustin, W. D. 2005. Perbedaan Khasiat Antibakteri Bahan Irigasi Antara Hydrogen Peroksida 3% Dan Infusum Daun Sirih 20% Terhadap Bakteri Mix. Dent. J, 38(1): 45-47.

Andayani, T., Y. Hendrawan, R. Yulianingsih. 2014. Minyak Atsiri Daun Sirih Merah (Piper crotatum) sebagai Pengawet Alami pada Ikan Teri (Stolephorus indicus). Jurnal Bioproses Komoditas Tropis. 2(2): 123-130.

Centers for Disease Control and Preventio/CDC. 2016. Disinfection and Sterilization: Introduction, Methods, Definition of Term. National Center for Emerging and Zoonotic Infectious Desease (NCEZID), Division of Healthcare Quality Promotion. U.S. Departement of Health & Human Servive. https://www.cdc.gov/infectioncontrol/guidelines/disinfection/introduction.html

Davis dan Stout. 1971. Disc Plate Method of Microbiological Antibiotic Essay. Journal of Microbiology. Vol 22 No 4.

Dudeja, P and A. Singh. 2017. Food safety in large organized eating establishments in: Food Safety in the 21st Century. P. 339-353. Academic Press. https://doi.org/10.1016/B978-0-12-801773-9.00027-3

Drauch, V., C. Ibesich, C. Volg, M. Hess, C. Hess. 2020. In-Vitro testing of bacteristatic and bactericidal efficacy of commercial disinfectant againt Salmonella Infantis reveals substantial differences between products and bacterial strains. International Journal of Food Microbiology 328 (2020) 108660.

Featherstone, C.A., R. Reichel, L.C. Snow, R.H. Davies, K.H. Christiansen, J.J. Carrique-Mas, S.J. Evans. 2010. Investigations of risk factors for Salmonella on fattening-turkey farms. Epidem. Infect., 138 (2010) : 1427-1438.

Gustina, I., Agustin, dan Widiana, Rina. 2012. Daya Hambat Daun Sirih Merah (Piper crocatum Ruiz & Pav) terhadap Pertumbuhan Bakteri Escheria coli dan Staphylococcus aureus. Padang: Jurnal Sainstek. Vol. IV, No. 2: 141-144.

Huguenin, J.E and J. Colt. 2002. Design and Operating Guide for Aquaculture Seawater Systems. In: Developments in Aquaculture and Fisheries Science. 2nd Eds. Vol. 33 p. 183-192. https://doi.org/10.1016/S0167-9309(02)80013-8

Haryadi, R. B. E. 2010. Daya Antibakteri Daun Sirih (Piper Betle) dan Daun Sirih Merah (Piper crocatum) terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro Sebagai Materi Praktikum Mikrobiologi. Tesis Pendidikan Biologi Program Pascasarjana Universitas Negeri Malang.

IBM. 2014. SPSS Software. IBM.com

Kartika, E.P., Marchaban, Sudarsono. 2018. Aktivitas Antibakteri Minyak Sirih Merah (Piper crotatum Ruiz & Pav.) Dalam Bentuk Sediaan Emulsi dan Mikroemulsi. Majalah Farmaseutik 14(2): 79-86.

Kushwaha, A dan G.B. P. Nagar. 2012. Food Preservation and Storage : Classification of Food Based on Perishability. http://ecoursesonline.iasri.res.in/mod/page/view.php?id=19549. Diakses 27 Agustus 2021.

Lukman, D.W., R. R. Soejoedono. 2009. Uji Sanitasi Dengan Metode RODAC Penuntun Praktikum Higiene Pangan Asal Ternak. Bagian Kesehatan Masyarakat Veteriner. Departemen Ilmu Penyakit Hewan dan Kesmavet. Fakultas Kedokteran Hewan. Institut Pertanian Bogor. Bogor.

Marlina, E.T., E. Harlia, Y.A. Hidayati, D.Z. Badruzzaman, W. Juanda. 2020. Pengaruh Penggunaan Kulit Lidah Buaya sebagai Disinfektan Alami terhadap Daya Hambat di Ruang Penampungan Susu. Jurnal Ilmu Ternak20(2): 158-163. DOI: 10.24198/jit.v20i2.31798

UHD. 2019. Classification of Perishable Foods. University of Houston Downtown. https://www.uhd.edu/administration/environmental-health-safety/Pages/Classification-of-Perishable-Foods.aspx. Diakses 27 Agustus 2021.

Retno, S. dan Dewi. 2006. Studi Efektivitas Sediaan Gel Antiseptik Tangan Esktrak Daun Sirih : I. Studi Formulasi, Laporan Penelitian, Fakultas Farmasi, Universitas Airlangga Surabaya.

Rizkita, A.D., E. Cahyono, S. Mursiti. 2017. Isolasi dan Uji Antibactri Minyak Atsiri Hijau dan Merah terhadap Streptococcus mutans. Indo. J. Chem. Sci. 6(3): 279-286.

Soleha, T.U., N. Carolia, S.W. Kurniawan. 2015. The inhibition test of red betel leaves (Piper crotatum) towards Staphylococcus aureus and Salmonella typhi. J. Majoruty 4(5): 117-122.

Saraswati, A. 2018. Analisis Kualitatif dan Kuantitatif Minyak Atsiri Sirih Hijau (Piper betle L.) dan Daun Sirih Merah (Piper crocatum Ruiz & Pav.) Berasal dari Kupang NTT. Calyptra: Jurnal Ilmiah Mahasiswa Universitas Surabaya. 7(2): 1640-1659.

Syafriana, V. dan R. Rusyita. 2017. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirih Merah (Piper crocatum) terhadap Pertumbuhan Propionibacterium acnes. Sainstech Farma 10 (2): 9-11.

Stefanny, G. 2010. Mekanisme Daya Hambat Kombinasi Ekstrak Daun Sirih Hijau (Piper Betle linn.) Dan Ekstrak Daun Sirih Merah (Piper Crocatum) Terhadap Pertumbuhan Candida Albicans. Skripsi. Fakultas Kedokteran Gigi Universitas Airlangga Surabaya.

Sujuno, H., S. Rizal, S. Purbaya, Jasmansyah. 2019. Uji Aktivitas Antibakteri Minyak Atsiri Daun Sirih Hijau (Piper betle L.) terhadap Bakteri Streptococcus pyogenes dan Staphylococcus aureus). J. Kartika Kimia. 2(1):30-36. DOI: https://doi.org/10.26874/jkk.v2i1.27

Surjowardojo, P., T.E. Susilorini, G.R.B. Sirait. 2015. Daya Hambat Dekok Kulit Apel Manalagi (Malus sylvestrs Mill.) terhadap Pertumbuhan Staphylococcus aureus dan Pseudomonas sp. Penyebab Mastitis pada Sapi Perah. J. Ternak Tropika 16(2): 40-48.

Soedibjo, M. 1991. Alam Sumber Kesehataan Manfaat dan Penggunaan. Balai Pustaka, Jakarta.

Tao, M., T. Ao, X. Mao, X. Yan, R. Javed, W. Hou, Y. Wang, C. Sun, S. Lin, T. Yu, Q.Ao. 2021. Sterilizationand disinfection methods for decellularized matrix materials: Review, consideration and proposal. Bioactive Materials. 6 (2021): 2927-2945. https://doi.org/10.1016/j.bioactmat.2021.02.010

Van Asselt, A.J.; Te Giffel, M.C. 2009. Hygienic practices in liquid milk dairies. In Market Milks–Processing and Quality Management; Tamime, A.Y., Ed.; Blackwell Publishing: Oxford, UK; pp. 48–71.

Volk, W. A dan M. F. Wheeler. 1990. Mikrobiologi Dasar. Terjemahan Adisoemarto. Edisi ke-5. Penerbit Erlangga, Jakarta.

Widiatuti, D. I. F. Karima, E. Setiyani. 2019. Efek Antibakteri Sodium Hypochlorite terhadap Staphylococcus aureus. Jurnal Ilmiah Kesehatan Masyarakat 11(4): 295-302.

Winiati, P. Rahayu. 2008. Keamanan Pangan dan Peduli Kita Bersama. Penebar Swadaya – IPB Press. Bogor.

Xie, Y., W. Yang, F. Tang, X. Chen, L. Ren. 2015. Antibacterial Activities of flavonoids: Structure-Avtivity Relationship and Mechanism. Current Medical Chemistry 22(1): 132-149.

Yonata, Q.U., I. Thohari, Marlik. 2020. Faktor yang berhubungan dengan angka kuman udara di Rumah Sakit Soemitro Surabaya. Jurnal Penelitian Kesehatan Suara Forikes 11 (3): 264-266.




DOI: http://dx.doi.org/10.31602/zmip.v47i1.5450

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

 

    

 

 

This work is licensed under a Creative Commons Attribution 4.0 International License.