ANALISIS FILOGENETIK GARCINIA SPP. BERDASARKAN SEKUENS GEN rRNA
(1) Universitas Lambung Mangkurat
(2) Universitas Lambung Mangkurat
(*) Corresponding Author
Sari
Garcinia genus has a complicated taxonomy due to the high similarities in morphological characters of its members. The phylogenetic analysis on Garcinia species based on rRNA gene sequence intended to find out the evolutionary relationship amongst the species. This research employed 20 sequences of the rRNA gene of Garcinia species selected from the GenBank of the National Center for Biology Information (NCBI). The sequences were aligned using ClustalW with the MEGA X application. A phylogenetic tree was constructed Maximum Likelihood method approximation and Kimura 2-parameter model. The results of the analysis showed that the cladogram had monophyletic parameter properties and classified into 3 clades. Clade I consisted of G. celebica, G. hombroniana, G. opaca, G. mangostana, G. malaccensis, G. penangiana, G. scortechinii, G. hanburyi, and G. urophylla. Clade II included G. atroviridis, G. bancana, G. forbesii, G. griffithii, G. cowa, G. nigrolineata, G. globulosa, and G. parvifolia. Clade III composed of G. rostrata, G. nervosa, and G. praininiana. The species of Garcinia considered the most primitive and the closet to their ancestor is G. nervosa.
Kata Kunci
Teks Lengkap:
PDFReferensi
Dharmayanti, N. L. P. I. 2011. Filogenetika Molekular : Metode Taksonomi Organisme Berdasarkan Sejarah Evolusi. WARTAZOA. 21(1), 1-10.
Elela, S. A., & Nazar, R. N. 1997. Peran rRNA 5,8S dalam Translokasi Ribosom. Penelitian Asam Nukleat. 25(9), 1788-1794.
Fitmawati, A. Swita, N. Sofyanti., & Herman. 2013. Analisis Kekerabatan Morfologi Mangifera dari Sumatera Tengah. Floribunda. 4(7), 169-174.
Hujjatusnaini, N. 2016. Konservasi Kawasan Hutan Di Lamandau dengan Konsep Bioremiadiasi dan Adat Dayak Kaharingan. Jurnal BIOEDUKASI. 4(2), 498-510.
Lim T.K., 2012. Garcinia forbesii. In: Edible Medicinal And Non-Medicinal Plants. Springer, Dordrecht
Mello, B. 2018. Estimating TimeTrees with MEGA and the TimeTree Resource. Mol. Biol. Evol. 35(9):2334–2342
Nei, M., & Kumar, S. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
Noor, M., M. Saleh., & H. Subagio. 2015. Potensi Keanekaragaman Tanaman Buah-Buahan di Lahan Rawa dan Pemanfaatannya. PROS SEM MASY BIODIV INDON. 1(6), 1348-1358. https://doi.org/10.13057/psnmbi/m010615
Putri, M. A. 2015. Variasi Genetik Rotan Berdasarkan Penanda DNA Barcode matK, rbcL, dan ITS Pada Pangkalan Data Genbank. Skripsi. Institut Pertanian Bogor, Bogor.
Santoso, P. J., E. Mansyah., & A. Pancoro. 2019. Klarifikasi Keanggotaan Manggis ‘Malinau’ Dalam Species Garcina mangostana Menggunkan Analisis Filogenik. Jurnal Pertanian Tropik. 6(1), 43-48.
Sari, R., & M. Gafurhan. 2013. Manggis Membulat Telur dari Kalimantan Barat. Floribunda. 4(6), 154-159.
Sinaga, S., Sobir, R. Poerwanto, H. Aswidinnoor., & D. Duryadi. 2010. Genetic Diversity and The Relationship Between The Indonesian Mangosteen (Garcinia mangostana) and The Related Species Using Isozyme Markers. Jurnal Natur Indonesia. 13(1), 53-58. https://doi.org/10.31258/jnat.13.1.53-58
Tamura K, J. Dudley, M. Nei & S. Kumar. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24:1596-1599.
Te-Chato, S. 2007. Floral and Fruit Morphology of Some Species In Garcinia spp. Songklanakarin Journal of Science and Technology. 29(2), 245-252.
Uji, T. 2007. Keanekaragaman, Persebaran, dan Potensi Jenis-Jenis Garcinia di Indonesia. Hayati. 12, 129-135.
DOI: http://dx.doi.org/10.31602/zmip.v46i2.4526
Refbacks
- Saat ini tidak ada refbacks.
##submission.copyrightStatement##
